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The isolation of human endothelial cells Isolation requires specific technique for its optimal growth and maintaining of exact physiological features. Primary Human Endothelial Cells Isolationtechnique involves a meticulous stepwise process for maintaining its cellular purity, integrity, function and avoiding cellular mutation. Enzymatic dissociation of the adherent cells, selective dissociation techniques, and immunomagnetic sorting helps in ensuring correct isolation of the endothelial cells. Some of the endothelial markers that enable identification of healthy endothelial cells and isolation from surrounding stromal and immune cells include CD31, VEGFR2, and CD144. It is important to maintain sterile conditions, optimized exposure to enzymatic treatment, and controlled temperature. Maintaining a high precision isolation process enables researchers to gain native morphology, angiogenic properties and barrier properties.
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